C3010-0500 RPMI 1640 With L-Glutamine
500ml
【Product Description】
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
Ingredients mg/L
INORGANIC SALTS
Calcium chloride dihydrate 100.000
Magnesium sulphate anhydrous 48.840
Potassium chloride 400.000
Sodium chloride 6000.000
Sodium dihydrogen phosphate anhydrous 800.000
AMINO ACIDS
Glycine 10.000
L-Arginine hydrochloride 241.000
L-Asparagine 50.000
L-Aspartic acid 20.000
L-Cystine dihydrochloride 65.200
L-Glutamic acid 20.000
L-Glutamine 300.000
L-Histidine hydrochloride monohydrate 20.960
L-Hydroxyproline 20.000
L-Isoleucine 50.000
L-Leucine 50.000
L-Lysine hydrochloride 40.000
L-Methionine 15.000
L-Phenylalanine 15.000
L-Proline 20.000
L-Serine 30.000
L-Threonine 20.000
L-Tryptophan 5.000
L-Tyrosine Disodium Salt 28.830
L-Valine 20.000
VITAMINS
Choline chloride 3.000
D-Biotin 0.200
D-Ca Pantothenate 0.250
Folic acid 1.000
Niacinamide 1.000
Pyridoxal hydrochloride 1.000
Riboflavin 0.200
Thiamine hydrochloride 1.000
Vitamin B1 20.005
i-Inositol 35.000
p-Amino benzoic acid (PABA) 1.000
OTHERS
D-Glucose 2000.000
Glutathione reduced 1.000
Phenol red sodium salt 5.300
Sodium bicarbonate 2000.000
【Storage and Stability】
RPMI 1640 , with L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/granulation/particulates/precipitates or sediments ;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1. Take a bottle from the defined storage conditions at 2-8°C and read the label.
2. Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3. Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4. Antibiotics may be added if desired.
【Quality Control】
RPMI 1640, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.